This is how I prepare my single colonies for winter. I just finished OA sublimation, the mouse guard is on the entrance, shim is added with a sugar mixture(emergency feed and maybe moisture control?), Super DFM probiotic, then insulated box cover. As you see most still also have pollen substitutes also. I have been using this procedure for years on over 100 colonies a year with great results. We also use it for 5 frame doubles and 8 frame singles.